DNA Extraction and Purification
Macherey-Nagel MN Square-well Block, 24 blocks
MN Square-well Block (24) 96-well blocks with 2.1 mL square wells pack of 24
Alkali Scientific Radiant™ Extract & Amplify Tissue PCR Kit 80 Reactions 2 x 1mL 2X HS Taq Red, 1 x 1.6mL 5X Extract Buffer A, 1 x .08mL 10X Extract Buffer B
Radiant™ Extract and Amplify Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tail, ear punch and other mammalian tissues, buccal swabs, hair shafts, and saliva. SPEED UP your GENOTYPING NO OVERNIGHT lysing Protocol: Extraction Solutions, included: 15 minutes then PCR as directed with supplied Radiant™ RED 2X mix During the quick 5 minute extraction process, DNA is released from the starting material by mixing the sample with ASI's proprietary Extraction Solution. This is followed by a 10 minute incubation at 95 degree to help inactivate proteins and genomic DNA. NO OVERNIGHT EXTRACTION. There is no need for mechanical disruption, organic extraction, column purification, or precipitation of the DNA.
Revvity Health Sciences Inc Microbiome Homogenizing Mix 2.8 and 0.1 mm Ceramic (2 mL Tubes) - Microbial DNA Free (50/pk)
Microbiome Homogenizing Mix 2.8 and 0.1 mm Ceramic (2 mL Tubes) - Microbial DNA Free (50/pk)
Macherey-Nagel NucleoSpin Plasmid Columns, 250 columns
NucleoSpin Plasmid Columns (250) binding columns for the isolation of plasmid DNA pack of 250
Macherey-Nagel MACHEREY-NAGEL NucleoSpin RNA Kit, High-Yield RNA Isolation, On-Column rDNase Digest, 250 Preps
The MACHEREY-NAGEL NucleoSpin RNA Kit is an efficient RNA isolation kit that facilitates the purification of high-quality RNA from various sample types, including cells and tissues. With an included on-column rDNase for DNA removal and quick preparation time, it streamlines workflows for sensitive applications like RT-PCR and RNA-sequencing. Its silica membrane technology ensures reliable results, making it a standout choice for scientists needing effective and dependable RNA purification.
Macherey-Nagel NucleoSpin 96 PCR Clean-up, 4 x 96 preps
NucleoSpin 96 PCR Clean-up (4x96) 4x 96 preps for the purification of PCR products - NucleoSpin PCR Clean-up Binding Plates, Elution Plates U-bottom, Self-adhering Foils, MN Wash Plates, buffers
INTACT GENOMICS INC RNA Cleanup Kit 25 Preps
The Intact Genomics IG RNA Cleanup Kit purifies up to 50 µg of RNA. Has a rapid and simplified workflow with a single wash buffer. Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt;)Option to Elute in 20-50 µL for more concentrated RNA than the standard 100 µL; Standard 70-100% RNA recovery; Customizable buffer options for difficult reactions; Purified RNA is ready for use in a wide variety of downstream applications including: RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, and transfection.This kit is recommended for use following the IG sgRNA Synthesis Kits to remove proteins, salts and most unincorporated nucleotides. The Intact Genomics RNA Cleanup Kit has a simplified workflow and is ideal for cleanup and concentration of RNA after enzymatic treatments such as DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT).
Biotium RNase-Free Calf Thymus DNA, 1 mg/mL (1 mL)
During isolation of DNA, RNase treatment is performed to eliminate contaminating RNA. Because RNase activity is robust and resistant to denaturation, commercial preparations of DNA contain high levels of residual RNase activity, even after re-purification. Preparing DNA solutions in DEPC-treated water will not inactivate RNase already present in the DNA. Similarly, commercial RNase inhibitor additives are usually poorly effective, expensive, and interfere in downstream applications. Biotium has developed a unique proprietary method that irreversibly inactivates RNases in purified DNA. The resulting DNA solution is free of excess protein or other chemicals that may interfere with downstream applications. Our RNase-free Calf Thymus DNA solution undergoes stringent quality control testing to confirm the product is 100% free of RNase activity. The solution is provided as 1 mg/mL calf thymus DNA in TE. Store the product at 4⁰C.
Zymo Research Corporation Quick-DNA/RNA Viral Kit (2x96)
The kit also features a buffer system that facilitates complete viral particle lysis for efficient nucleic acid isolation. Small (> 50 nt) and large (> 200 kb) DNA and RNA are bound to each well of the plate, washed and eluted.The isolated high-quality nucleic acids are ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/qPCR detection.
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (800 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
ATCC Quantitative Genomic; DNA from Giardia; intestinalis strain; Portland-1
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine protozoan load.